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Human rhinovirus 19
Human rhinovirus 19
規(guī)格:
貨期:
編號(hào):B162804
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) Human rhinovirus 19
商品貨號(hào) B162804
Classification Picornaviridae, Enterovirus, Rhinovirus A
Agent Human rhinovirus 19
Strain 6072 [6072-CV 19]
Applications Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Comments ATCC® 1929™ was derived from ATCC® 494™ following treatment with diethyl ether to remove Mycoplasma to remove mycoplasma contamination.
Effect on Host
CPE, rounding and sloughing
Recommended Host
EMEM (ATCC® 30-2003™) + 2% FBS (ATCC® 30-2020™)
Growth Conditions

Temperature: 33.5°C

Recommendations for Infection: Plate cells 16-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33.5°C in a humidified 5% CO2 atmosphere, rocking continuously. End adsorption by adding virus growth medium.

Incubation: 2-4 days at 33.5°C in a humidified 5% CO2 atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer.

Name of Depositor VV Hamparian, RM Conant
Source Human throat washings
References

Ketler A, et al. Characterization and classification of ECHO 28-rhinovirus-coryzavirus agents. Proc. Soc. Exp. Biol. Med. 110: 821-831, 1962. PubMed: 14455433

Palmerberg AC and Gern JE. Classification and evolution of human rhinoviruses. Methods Mol Biol. 1221:1-10, 2015. doi: 10.1007/978-1-4939-1571-2_1. PubMed: 25261302

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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