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A5.4 [1506 A5.4]
A5.4 [1506 A5.4]
規(guī)格:
貨期:
編號(hào):B163885
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 A5.4 [1506 A5.4]
商品貨號(hào) B163885
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds to an epitope in amino acids 113 through 243 on human ApoA-I.
It is useful for detecting ApoA-I in immunoblotting applications.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human apolipoprotein A-I (Apo-A-I)
Cellular Products
immunoglobulin; monoclonal antibody; against human apolipoprotein A-I (Apo-A-I)
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with the major protein peak (G-200 fractionation) of delipidated human high density lipoprotein (HDL).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to an epitope in amino acids 113 through 243 on human ApoA-I.
It is useful for detecting ApoA-I in immunoblotting applications.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate, 85%; horse serum, 10%; fetal bovine serum, 5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG2a
Name of Depositor G Schonfeld
Deposited As mouse (B cell); mouse (myeloma)
References

Jonas A, et al. Apolipoprotein A-I structure and lipid properties in homogeneous, reconstituted spherical and discoidal high density lipoproteins. J. Biol. Chem. 265: 22123-22129, 1990. PubMed: 2125044

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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