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AF6-122.2.5
AF6-122.2.5
規(guī)格:
貨期:
編號(hào):B163917
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 AF6-122.2.5
商品貨號(hào) B163917
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with cells bearing the b, e and o haplotypes at the Igh-C locus.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Igh-5.3 (IgD b,e)
Cellular Products
immunoglobulin; monoclonal antibody; against Igh-5.3 (IgD b,e)
Comments
Animals were immunized with spleen cells from C57BL/6 mice.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with cells bearing the b, e and o haplotypes at the Igh-C locus.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.
    Isotype IgG1
    Name of Depositor AM Stall
    Deposited As mouse (B cell); mouse (myeloma)
    References

    Stall AM, Loken MR. Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies. J. Immunol. 132: 787-795, 1984. PubMed: 6690619

    梅經(jīng)理 17280875617 1438578920
    胡經(jīng)理 13345964880 2438244627
    周經(jīng)理 17757487661 1296385441
    于經(jīng)理 18067160830 2088210172
    沈經(jīng)理 19548299266 2662369050
    李經(jīng)理 13626845108 972239479
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